Review
BibTex RIS Cite

Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için nasıl hazırlanır?

Year 2018, Volume: 11 Issue: 1, 27 - 32, 26.12.2018

İlknur Dağ

Abstract

Geçirimli Elektron Mikroskopları (TEM) hücre
biyolojisi alanında ve biyomedikal araştırmalarda çok önemli veriler sunan
araçlardır. Yeni teknolojilerin de geliştirilmesiyle örneğin daha iyi
görüntülenmesi sağlanmakta; böylece hem biyolojik örneğe ait bilgilerimiz
artmakta hem de önemli moleküllerin yerleşimi hakkında detaylı verilere
ulaşmamız mümkün olabilmektedir. Günümüze kadar pek çok mikroskop tipi
geliştirilmesine rağmen, elektron mikroskoplarının en büyük avantajı
çözünürlüklerinin çok yüksek olması ve hücre mimarisi hakkında çok detaylı
bilgiler sunabilmesidir. Örneğin canlı haline en yakın biçimde
görüntülenebilmesi için yapısal komponentlerinin iyi korunmuş olması gerekir ve
amaca uygun hazırlık protokülünün belirlenmesi ve uygulanması esastır. Bu
derlemede biyolojik örneklerin TEM ile görüntülenebilmesi için en sık
kullanılan teknikler hakkında genel bir bakış açısı sunulması hedeflenmiştir.
Geleneksel metodların yanı sıra en yeni kullanılan kriyo tekniklere de değinilmiş
ve her tekniğin avantaj, dezavantaj ve sunduğu bilgiler detaylandırılmıştır. 

Keywords

geçirimli elektron mikroskobu

References

  • KAYNAKLAR 1. Thompson RF, Walker M, Siebert CA, Muench SP, Ranson NA. An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology. Methods, 2016; 100,, 3-15.
  • 2. Park CH, Kim HW, Uhm CS. How to Get Well-Preserved Samples for Transmission Electron Microscopy, Applied Microscopy, 2016; 46 (4),188-192.
  • 3. Frankl A, Mari M, Reggiori F. Electron microscopy for ultrastructural analysis and protein localization in Saccharomyces cerevisiae. Microb Cell 2015, 2(11), 412–428.
  • 4. Wang H. Cryo-electron microscopy for structural biology: current status and future perspectives. Sci China Life Sci, 2015; 58(8), 750–756.
  • 5. Taylor KA, Glaeser RM. Retrospective on the early development of cryoelectron microscopy of macromolecules and a prospective on the opportunities for the future. Journal of Structural Biology, 2008. 163(2008), 214–223.
  • 6. Uhm CS, Park EK, Park CH. Tissue preparation with t-Butyl alcohol freeze-drying method for scanning electron microscopy: application for rat liver. Korean J Electron Microsc. 1998, 28, 299-306.
  • 7. Hayat MA. Principles and Techniques of Electron Microscopy: Biological Applications. 1989 CRC Press, Boca Raton, FL.
  • 8. Wisse E, Braet F, Duimel H, Vreuls C, Koek G, Steven WM, Damink O, Broek MAJ, Geest BD, Dejong CHC, Tateno C, Frederik P. Fixation methods for electron microscopy of human and other liver. World J Gastroenterol. 2010, 16 (23), 2851–2866.
  • 9. Mascorro JA, Bozzola JJ. Processing Biological Tissues for Ultrastructural Study. In: Kuo J. (eds) Electron Microscopy Methods in Molecular Biology™, 2007, 369, 19-34 Humana Press
  • 10. Bozola JJ, Russell LD. Electron Microscopy: Principles and Techniques forBiologists. second edition. 1992 John and Bartlett Publishers, Sudbury, Massachusetts, USA.
  • 11. Taylor KA, Glaeser RM. Electron diffraction of frozen, hydrated protein crystals. Science 1974, 186(4168), 1036–37.
  • 12. Adrian M, Dubochet J, Lepault J, McDowall AW. Cryo-electron microscopy of viruses. Nature 1984, 308, 32–36.
  • 13. Costello MJ. Cryo-electron microscopy of biological samples.Ultrastruct Pathol. 2006, 30(5),361-71.
  • 14. Studer D, Graber W, Al‐Amoudi A, Eggli PA. A new approach for cryofixation by high‐pressure freezing. J Microsc 2001, 203(3), 285–294
  • 15. Carlemalm E, Garavito M, Villiger W. Resin development for electron microscopy and an analysis of embedding at low temperature. J Microsc, 1982, 126 (2), 123-143.
  • 16. Tokuyasu KT. A technique for ultracryotomy of cell suspensions and tissues. J Cell Biol. 1973, 57(2), 551–565.
  • 17. Mobius W. Cryopreparation of biological specimens for immunoelectron microscopyAnn. Anat-Anat. Anz., 2009, 191(3),231-247
  • 18. Terracio L, Schwabe, KG. Freezing and drying of biological tissue for electron microscopy. J Histochem Cytochem. 1981, 29(9), 1021–8.
  • 19. Giddings TH. Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. J. Microsc. 2003, 212(pt1), 53-61
  • 20. Mcdonald KL. A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues. Journal of microscopy 2009, 235(3), 273–281.
  • 21. Ripper D, Schwarz H, Stierhof YD. Cryo-section immunolabelling of difficult to preserve specimens: advantages of cryofixation, freeze-substitution and rehydration. Biol. Cell 2008, 100(2), 109-123.
  • 22. Cavalier A, Spehner D, Humbel BM. Handbook of Cryo-Preparation Methods for Electron Microscopy. (Eds.). 2009,CRC Press, Boca Raton, FL;
  • 23. Edelmann L: Freeze-dried and resin-embedded biological material is well suited for ultrastructure research. J Microsc 2002, 207(1), 5–26
  • 24. Al-Amoudi A1, Chang JJ, Leforestier A, McDowall A, Salamin LM, Norlén LP, Richter K, Blanc NS, Studer D, Dubochet J. Cryo-electron microscopy of vitreous sections. EMBO J. 2004, 23(18), 3583-8.

Year 2018, Volume: 11 Issue: 1, 27 - 32, 26.12.2018

İlknur Dağ

Abstract

References

  • KAYNAKLAR 1. Thompson RF, Walker M, Siebert CA, Muench SP, Ranson NA. An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology. Methods, 2016; 100,, 3-15.
  • 2. Park CH, Kim HW, Uhm CS. How to Get Well-Preserved Samples for Transmission Electron Microscopy, Applied Microscopy, 2016; 46 (4),188-192.
  • 3. Frankl A, Mari M, Reggiori F. Electron microscopy for ultrastructural analysis and protein localization in Saccharomyces cerevisiae. Microb Cell 2015, 2(11), 412–428.
  • 4. Wang H. Cryo-electron microscopy for structural biology: current status and future perspectives. Sci China Life Sci, 2015; 58(8), 750–756.
  • 5. Taylor KA, Glaeser RM. Retrospective on the early development of cryoelectron microscopy of macromolecules and a prospective on the opportunities for the future. Journal of Structural Biology, 2008. 163(2008), 214–223.
  • 6. Uhm CS, Park EK, Park CH. Tissue preparation with t-Butyl alcohol freeze-drying method for scanning electron microscopy: application for rat liver. Korean J Electron Microsc. 1998, 28, 299-306.
  • 7. Hayat MA. Principles and Techniques of Electron Microscopy: Biological Applications. 1989 CRC Press, Boca Raton, FL.
  • 8. Wisse E, Braet F, Duimel H, Vreuls C, Koek G, Steven WM, Damink O, Broek MAJ, Geest BD, Dejong CHC, Tateno C, Frederik P. Fixation methods for electron microscopy of human and other liver. World J Gastroenterol. 2010, 16 (23), 2851–2866.
  • 9. Mascorro JA, Bozzola JJ. Processing Biological Tissues for Ultrastructural Study. In: Kuo J. (eds) Electron Microscopy Methods in Molecular Biology™, 2007, 369, 19-34 Humana Press
  • 10. Bozola JJ, Russell LD. Electron Microscopy: Principles and Techniques forBiologists. second edition. 1992 John and Bartlett Publishers, Sudbury, Massachusetts, USA.
  • 11. Taylor KA, Glaeser RM. Electron diffraction of frozen, hydrated protein crystals. Science 1974, 186(4168), 1036–37.
  • 12. Adrian M, Dubochet J, Lepault J, McDowall AW. Cryo-electron microscopy of viruses. Nature 1984, 308, 32–36.
  • 13. Costello MJ. Cryo-electron microscopy of biological samples.Ultrastruct Pathol. 2006, 30(5),361-71.
  • 14. Studer D, Graber W, Al‐Amoudi A, Eggli PA. A new approach for cryofixation by high‐pressure freezing. J Microsc 2001, 203(3), 285–294
  • 15. Carlemalm E, Garavito M, Villiger W. Resin development for electron microscopy and an analysis of embedding at low temperature. J Microsc, 1982, 126 (2), 123-143.
  • 16. Tokuyasu KT. A technique for ultracryotomy of cell suspensions and tissues. J Cell Biol. 1973, 57(2), 551–565.
  • 17. Mobius W. Cryopreparation of biological specimens for immunoelectron microscopyAnn. Anat-Anat. Anz., 2009, 191(3),231-247
  • 18. Terracio L, Schwabe, KG. Freezing and drying of biological tissue for electron microscopy. J Histochem Cytochem. 1981, 29(9), 1021–8.
  • 19. Giddings TH. Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. J. Microsc. 2003, 212(pt1), 53-61
  • 20. Mcdonald KL. A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues. Journal of microscopy 2009, 235(3), 273–281.
  • 21. Ripper D, Schwarz H, Stierhof YD. Cryo-section immunolabelling of difficult to preserve specimens: advantages of cryofixation, freeze-substitution and rehydration. Biol. Cell 2008, 100(2), 109-123.
  • 22. Cavalier A, Spehner D, Humbel BM. Handbook of Cryo-Preparation Methods for Electron Microscopy. (Eds.). 2009,CRC Press, Boca Raton, FL;
  • 23. Edelmann L: Freeze-dried and resin-embedded biological material is well suited for ultrastructure research. J Microsc 2002, 207(1), 5–26
  • 24. Al-Amoudi A1, Chang JJ, Leforestier A, McDowall A, Salamin LM, Norlén LP, Richter K, Blanc NS, Studer D, Dubochet J. Cryo-electron microscopy of vitreous sections. EMBO J. 2004, 23(18), 3583-8.
There are 24 citations in total.

Details

Primary Language Turkish
Journal Section Collection
Authors

İlknur Dağ

Publication Date December 26, 2018
Published in Issue Year 2018 Volume: 11 Issue: 1

Cite

APA Dağ, İ. (2018). Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için nasıl hazırlanır?. Türk Bilimsel Derlemeler Dergisi, 11(1), 27-32.
AMA Dağ İ. Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için nasıl hazırlanır?. Turk.Bilimsel Derleme Derg. December 2018;11(1):27-32.
Chicago Dağ, İlknur. “Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için nasıl hazırlanır?”. Türk Bilimsel Derlemeler Dergisi 11, no. 1 (December 2018): 27-32.
EndNote Dağ İ (December 1, 2018) Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için nasıl hazırlanır?. Türk Bilimsel Derlemeler Dergisi 11 1 27–32.
IEEE İ. Dağ, “Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için nasıl hazırlanır?”, Turk.Bilimsel Derleme Derg., vol. 11, no. 1, pp. 27–32, 2018.
ISNAD Dağ, İlknur. “Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için nasıl hazırlanır?”. Türk Bilimsel Derlemeler Dergisi 11/1 (December 2018), 27-32.
JAMA Dağ İ. Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için nasıl hazırlanır?. Turk.Bilimsel Derleme Derg. 2018;11:27–32.
MLA Dağ, İlknur. “Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için nasıl hazırlanır?”. Türk Bilimsel Derlemeler Dergisi, vol. 11, no. 1, 2018, pp. 27-32.
Vancouver Dağ İ. Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için nasıl hazırlanır?. Turk.Bilimsel Derleme Derg. 2018;11(1):27-32.
 Download Cover Image