Research Article
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Türkiye’nin Bingöl ili Bal Arı Kolonilerinde Deforme kanat virüsü ve Kronik arı felci virüsü’nün Prevalansı

Year 2023, Volume: 13 Issue: 1, 44 - 53, 01.03.2023
https://doi.org/10.21597/jist.1059418

Abstract

Bal arılarını infekteleyen virüsler dünyanın her yerinde bal arılarının her gelişim evresinde
infeksiyona neden olan ve ekonomik zarara yol açan hastalıklardır. Bu araştırmada dünya
çapında yaygın olan iki bal arısı virüsünün (deforme kanat virüsü [DWV] ve kronik arı paraliz
virüsü [CBPV]) varlığı ve yaygınlığını belirlemek için bir survey çalışması gerçekleştirilmiştir.
Viral RNA’yı belirlemek amacıyla Bingöl ili sınırlarındaki 9 farklı lokaliteden toplam 128
arılık ziyaret edilmiştir. Toplanan 384 bal arısı örneği her virüse özgü genom spesifik primerler
kullanılarak moleküler olarak (revers-transkriptaz polimeraz zincir reaksiyonu, RT-PCR)
testlenmiştir. 128 arılığın 28’i (%21.87) DWV için pozitif reaksiyon vermiştir fakat hiçbir
arılıkta CBPV patojeni tespit edilmemiştir. Pozitif örneklerden rastgele seçilen birinin
klonlanarak ortaya çıkarılan 702 bazlık nükleotit dizisi gen bankasına MZ357973 ulaşım
numarasıyla kaydedilmiştir. NCBI veri tabanındaki BLASTn analizine göre, Bingöl ilinde
belirlenen DWV etmeninin nükleotit dizisi aynı virüsün diğer izolatlarıyla kıyaslandığında
%77.82-98.45 arasında değişen nükleotit benzerliği göstermiştir. Ayrıca, farklı orijinlerden
izolatların kullanıldığı filogenetik ağaca dayanarak bizim izolatımız Türkiye’den ErzincanDWV izolatıyla yakın filogenetik ilişki içerisinde olduğu tespit edilmiştir. Literatür
taramalarına göre, bu çalışma Bingöl ilinde DWV ve CBPV yaygınlığını ve varlığını DNA
temelli yöntemlerle ortaya koyan ilk kayıt niteliğindedir

Supporting Institution

bulunmamaktadır

Project Number

BAP-ZF.2020.00-004

Thanks

Araştırmada katkısı geçen herkese teşekkür ederim. Ayrıca yayın sürecinde emeği geçen sayın dergi editörü ve sekreteryasına da teşekkürlerimi bildiririm.

References

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Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province, Turkey

Year 2023, Volume: 13 Issue: 1, 44 - 53, 01.03.2023
https://doi.org/10.21597/jist.1059418

Abstract

Viruses infecting honey bees are diseases that cause infection and economic damage in every
developmental stage of honey bees all over the world. In this study, a survey was conducted to
determine the presence and prevalence of two worldwide common honeybee viruses (deformed
wing virus [DWV] and chronic bee paralysis virus [CBPV]). 128 apiaries from 9 different
localities in the province of Bingöl were visited to determine viral RNA. Collected 384 honey
bee samples were tested molecularly (reverse-transcriptase polymerase chain reaction, RTPCR) using genome-specific primers specific to each virus. In molecular tests, 28 of 128
apiaries (21.87%) gave a positive reaction for DWV, but no CBPV pathogen was detected in
any apiary. The 711 bp nucleotide sequence revealed by cloning one of the randomly selected
positive samples was registered in the gene bank with the accession number MZ357973.
According to the BLASTn analysis in the NCBI database, the nucleotide sequence of the DWV
agent determined in Bingöl Province showed nucleotide similarity between 77.82-98.45%
when compared to other isolates of the same virus. In addition, our isolate was found to be in a
close phylogenetic relationship with the Erzincan-DWV isolate from Turkey, based on the
phylogenetic tree using isolates from different origins. According to our literature screening,
this study is the first record that reveals the prevalence and the presence of DWV and CBPV in
Bingöl Province with DNA-based approaches.

Project Number

BAP-ZF.2020.00-004

References

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  • Ai, H., Yan, X., & Han, R. (2012). Occurrence and prevalence of seven bee viruses in Apis mellifera and Apis cerana apiaries in China. Journal of invertebrate pathology, 109(1), 160-164.
  • Allen MF, Ball BV, 1996. The incidence and world distribution of honey bee viruses. Bee World, 77: 141–162.
  • Antúnez, K., Anido, M., Garrido-Bailon, E., Botias, C., Zunino, P., Martínez-Salvador, A., ... & Higes, M. (2012). Low prevalence of honeybee viruses in Spain during 2006 and 2007. Research in Veterinary Science, 93(3), 1441-1445.
  • Avalos, J., Rosero, H., Maldonado, G., & Reynaldi, F. J. (2019). Honey bee louse (Braula schmitzi) as a honey bee virus vector?. Journal of Apicultural Research, 58(3), 427-429.
  • Ayşegül, U.S.T.A., & Yıldırım, Y. (2022). Investigation of deformed wing virus, black queen cell virus and acute bee paralysis virus infections by using reverse transcriptase-polymerase chain reaction (RT-PCR) technique in honey bees. Ankara Üniversitesi Veteriner Fakültesi Dergisi. https://doi.org/10.33988/auvfd.824882.
  • Bacandritsos, N., Granato, A., Budge, G., Papanastasiou, I., Roinioti, E., Caldon, M., ... & Mutinelli, F. (2010). Sudden deaths and colony population decline in Greek honey bee colonies. Journal of invertebrate pathology, 105(3), 335-340.
  • Bailey, L., Ball, B. V., & Perry, J. N. (1981). The prevalence of viruses of honey bees in Britain. Annals of Applied Biology, 97(1), 109-118.
  • Bailey L, Ball BV, 1991. Honeybee pathology. Academic Press, London, United Kingdom.
  • Berényi, O., Bakonyi, T., Derakhshifar, I., Köglberger, H., & Nowotny, N. (2006). Occurrence of six honeybee viruses in diseased Austrian apiaries. Applied and environmental microbiology, 72(4), 2414-2420.
  • Budge, G. E., Simcock, N. K., Holder, P. J., Shirley, M. D., Brown, M. A., Van Weymers, P. S., ... & Rushton, S. P. (2020). Chronic bee paralysis as a serious emerging threat to honey bees. Nature Communications, 11(1), 2164.
  • ÇAĞIRGAN, A. A., & Yazici, Z. (2021). The prevalence of seven crucial honeybee viruses using multiplex RT-PCR and theirphylogenetic analysis. Turkish Journal of Veterinary & Animal Sciences, 45(1), 44-55..
  • Cagirgan, A. A., Yildirim, Y., & Usta, A. (2020). Phylogenetic analysis of deformed wing virus, black queen cell virus and acute bee paralysis viruses in Turkish honeybee colonies. Medycyna Weterynaryjna, 76(08)..
  • Celle, O., Blanchard, P., Olivier, V., Schurr, F., Cougoule, N., Faucon, J. P., & Ribière, M. (2008). Detection of Chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread. Virus research, 133(2), 280-284..
  • Chen, G., Wu, Y., Deng, J., Wen, Z., Wang, S., Chen, Y., ... & Zheng, H. (2021). Seasonal variation of viral infections between the eastern honey bee (Apis cerana) and the western honey bee (Apis mellifera). Microbiologyopen, 10(1), e1162.
  • Chen, Y. (2011). Viruses and viral diseases of the honey bee, Apis mellifera. Recent Advances in Entomological Research: From Molecular Biology to Pest Management, 105-120.
  • Chen, Y., Smith, I. B., Collins, A. M., Pettis, J. S., & Feldlaufer, M. F. (2004). Detection of deformed wing virus infection in honey bees, Apis mellifera L., in the United States. American Bee Journal, 144(7), 557-559..
  • De Miranda, J. R., & Genersch, E. (2010). Deformed wing virus. Journal of invertebrate pathology, 103, S48-S61..
  • De Miranda, J. R., & Fries, I. J. J. O. I. P. (2008). Venereal and vertical transmission of deformed wing virus in honeybees (Apis mellifera L.). Journal of invertebrate pathology, 98(2), 184-189.
  • Desai, S. D., Eu, Y. J., Whyard, S., & Currie, R. W. (2012). Reduction in deformed wing virus infection in larval and adult honey bees (Apis mellifera L.) by double‐stranded RNA ingestion. Insect molecular biology, 21(4), 446-455.
  • Di Pasquale, G., Salignon, M., Le Conte, Y., Belzunces, L. P., Decourtye, A., Kretzschmar, A., ... & Alaux, C. (2013). Influence of pollen nutrition on honey bee health: do pollen quality and diversity matter?. PloS one, 8(8), e72016.
  • Dolezal, A. G., Hendrix, S. D., Scavo, N. A., Carrillo-Tripp, J., Harris, M. A., Wheelock, M. J., ... & Toth, A. L. (2016). Honey bee viruses in wild bees: viral prevalence, loads, and experimental inoculation. PloS one, 11(11), e0166190.
  • Eyer, M., Chen, Y. P., Schäfer, M. O., Pettis, J., & Neumann, P. (2009). Small hive beetle, Aethina tumida, as a potential biological vector of honeybee viruses. Apidologie, 40(4), 419-428.
  • Svanella-Dumas, L., Dulucq, M. J., Candresse, T., Gentit, P., & Foissac, X. (2000, July). Polyvalent detection of fruit tree tricho, capillo and foveaviruses by nested RT-PCR using degenerated and inosine containing primers (PDO RT-PCR). In XVIII International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops-Top Fruit Diseases 550 (pp. 37-44).
  • Forsgren, E., De Miranda, J. R., Isaksson, M., Wei, S., & Fries, I. (2009). Deformed wing virus associated with Tropilaelaps mercedesae infesting European honey bees (Apis mellifera). Experimental and Applied Acarology, 47, 87-97.
  • Gauthier, L., Tentcheva, D., Tournaire, M., Dainat, B., Cousserans, F., Colin, M. E., & Bergoin, M. (2007). Viral load estimation in asymptomatic honey bee colonies using the quantitative RT-PCR technique. Apidologie, 38(5), 426-435.
  • Genersch, E., & Aubert, M. (2010). Emerging and re-emerging viruses of the honey bee (Apis mellifera L.). Veterinary research, 41(6).
  • Güller, A., Mustafa, U. S. T. A., Çakar, G., & Zeynelabidin, K. U. R. T. (2021). Molecular characterization of Deformed wing viruses identified in honeybee (Apis mellifera L.) colonies in Erzincan province of Turkey. Avrupa Bilim ve Teknoloji Dergisi, (27), 186-192.
  • Gülmez, Y., Bursalı, A., & Tekin, S. (2009). First molecular detection and characterization of deformed wing virus (DWV) in honeybees (Apis mellifera L.) and mite (Varroa destructor) in Turkey. African Journal of Biotechnology, 8(16).
  • Okur Gumusova, S., Albayrak, H., Kurt, M., & Yazici, Z. (2010). Prevalence of three honey bee viruses in Turkey. Veterinarski Arhiv, 80(6), 779-785.
  • Haddad, N., Al-Gharaibeh, M., Nasher, A., Anaswah, E., Alammari, Y., & Horth, L. (2018). Scientific note: molecular detection of pathogens in unhealthy colonies of Apis mellifera jemenitica. Apidologie, 49(1), 84-88.
  • Haddad, N., Brake, M., Migdadi, H., & de Miranda, J. R. (2008). First detection of honey bee viruses in Jordan by RT-PCR. Jordan Journal of Agricultural Sciences, 4(3), 242-247.
  • Highfield, A., Kevill, J., Mordecai, G., Hunt, J., Henderson, S., Sauvard, D., ... & Schroeder, D. C. (2020). Detection and replication of Moku virus in honey bees and social wasps. Viruses, 12(6), 607.
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There are 60 citations in total.

Details

Primary Language English
Subjects Zootechny (Other)
Journal Section Bitki Koruma / Plant Protection
Authors

Adnan Balkaya This is me 0000-0002-8728-2092

Abdullah Güller 0000-0003-3887-4208

Mustafa Usta 0000-0002-3940-2774

Zeynelabidin Kurt 0000-0003-1164-9326

Project Number BAP-ZF.2020.00-004
Early Pub Date February 24, 2023
Publication Date March 1, 2023
Submission Date January 18, 2022
Acceptance Date October 25, 2022
Published in Issue Year 2023 Volume: 13 Issue: 1

Cite

APA Balkaya, A., Güller, A., Usta, M., Kurt, Z. (2023). Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province, Turkey. Journal of the Institute of Science and Technology, 13(1), 44-53. https://doi.org/10.21597/jist.1059418
AMA Balkaya A, Güller A, Usta M, Kurt Z. Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province, Turkey. J. Inst. Sci. and Tech. March 2023;13(1):44-53. doi:10.21597/jist.1059418
Chicago Balkaya, Adnan, Abdullah Güller, Mustafa Usta, and Zeynelabidin Kurt. “Prevalence of Deformed Wing Virus and Chronic Bee Paralysis Virus in Honey Bee Colonies of Bingöl Province, Turkey”. Journal of the Institute of Science and Technology 13, no. 1 (March 2023): 44-53. https://doi.org/10.21597/jist.1059418.
EndNote Balkaya A, Güller A, Usta M, Kurt Z (March 1, 2023) Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province, Turkey. Journal of the Institute of Science and Technology 13 1 44–53.
IEEE A. Balkaya, A. Güller, M. Usta, and Z. Kurt, “Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province, Turkey”, J. Inst. Sci. and Tech., vol. 13, no. 1, pp. 44–53, 2023, doi: 10.21597/jist.1059418.
ISNAD Balkaya, Adnan et al. “Prevalence of Deformed Wing Virus and Chronic Bee Paralysis Virus in Honey Bee Colonies of Bingöl Province, Turkey”. Journal of the Institute of Science and Technology 13/1 (March 2023), 44-53. https://doi.org/10.21597/jist.1059418.
JAMA Balkaya A, Güller A, Usta M, Kurt Z. Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province, Turkey. J. Inst. Sci. and Tech. 2023;13:44–53.
MLA Balkaya, Adnan et al. “Prevalence of Deformed Wing Virus and Chronic Bee Paralysis Virus in Honey Bee Colonies of Bingöl Province, Turkey”. Journal of the Institute of Science and Technology, vol. 13, no. 1, 2023, pp. 44-53, doi:10.21597/jist.1059418.
Vancouver Balkaya A, Güller A, Usta M, Kurt Z. Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province, Turkey. J. Inst. Sci. and Tech. 2023;13(1):44-53.