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Skrotal Radyoterapide Curcuminin Profilaktik Kullanımı Testis Dokusunda PARP-1 İmmünreaktivitesini ve Spermatogenezi Nasıl Etkiler?

Year 2018, Volume: 25 Issue: 4, 370 - 384, 01.12.2018
https://doi.org/10.17343/sdutfd.387496

Abstract



Fertilitede
akut doz radyasyonun öldürücü etkileri iyi bilinmektedir. Kısa süreli
radyasyona maruziyet germ hücrelerinin ölümü ve hasarlanması ile sonuçlanır.
Son yıllarda radyoterapi, kanserli hastaların uzun süreli iyileşmesine önemli
ölçüde katkı vermiştir. Ancak radyasyonun uzun dönemde üreme sistemi üzerindeki
hasarlayıcı etkisi ile meydana gelen fertilizasyon kayıplarını göz ardı etmemek
gerekmektedir. Bu güne kadar curcuminin antioksidan ve radyoprotektif etkisi
ile ilgili birçok makale yayınlanmıştır. Bu çalışma; curcuminin X ışınlamayı
takiben sıçan testisinde ortaya çıkacak germ hücre kaybı ile ilişkili infertiliteyi
iyileştirebileceği düşüncesi ile planlanmıştır.



Çalışmamızda biri kontrol 3’ü deney grubu olmak üzere
toplam 4 grup oluşturuldu. Radyasyon hasarı oluşturmak amacıyla kontrol ve
curcumin grubu dışındaki deneklerin skrotal bölgelerine tek fraksiyonda 6 Gy X
ışını uygulandı. Curcumin ve radyasyon+ curcumin gruplarına; ışınlamadan 1
hafta önce başlayarak, haftada 3 kez olmak üzere 7 hafta boyunca oral yoldan
100 mg/kg curcumin, kontrol ve radyasyon gruplarına da aynı şekilde serum
fizyolojik verildi. Işınlamadan 6 hafta sonra tüm denekler sakrifiye edildi ve
alınan testis biyopsi materyalleri histopatolojik incelemeler için
işlemlendirildi.



Çalışmamızda, iyonize radyasyonun tübüllerin çoğunda
germ hücre kaybına bağlı olarak, tübüler atrofiye neden olduğu görüldü. Radyasyon
alan deneklerin tümünde, spermatogenezisin bozulması ile ilişkili olarak, Jhonsen
skorunda kontrole göre anlamlı düzeyde (p<0,05) azalma saptandı. PARP-1
immünreaktivite skorunda da hasarının şiddeti ile ilişkili olarak, kontrole
göre anlamlı düzeyde (p<0,05) artış belirlendi. Curcumin uygulamasının ise
radyasyona bağlı ortaya çıkan hasarları önlemede beklenen düzeyde koruma ve
iyileşme sağlamadığı tespit edildi.

References

  • 10. Akpolat M, Kanter M, Uzal MC. Protective effects of curcumin against gamma radiation-induced ileal mucosal damage. Arch Toxicol 2009; 83(6): 609-617.
  • 11. Akpolat M, Topçu-Tarladaçalışır Y, Uz YH, Sapmaz-Metin M, Kızılay G. Kanser Tedavisinde Curcuminin Yeri. Yeni Tıp Dergisi 2010; 27: 142-147.
  • 12. Çimen Özgen S, Dökmeci D, Akpolat M, Karadağ ÇH, Gündüz Ö, Erbaş H, Benian Ö, Uzal C, Turan FN. The Protective Effect of Curcumin on Ionizing Radiation-induced Cataractogenesis in Rats. Balkan Med J 2012; 29: 358-363.
  • 13. Topçu-Tarladaçalışır Y, Akpolat M, Uz YH, Kızılay G, Sapmaz-Metin M, Cerkezkayabekir A, Kurt-Omurlu I. Effects of Curcumin on Apoptosis and Oxidoinflammatory Regulation in Rat Model of Acetic Acid-induced Colitis: Role of JNK and p38 MAPK. Journal of Medicinal Food 2013; 16(4): 296-305. 14. Schreiber V, Ame JC, Dolle P. Poly(ADP-ribose) polymerase-2 (PARP-2) is required for efficient base excision DNA repair in association with 78PARP-1 and XRCC1. J Biol Chem 2002; 277(25): 23028-23036.
  • 15. Meyer-Ficca ML, Meyer RG, Jacobson EL, Jacobson MK. Poly(ADP-ribose) polymerases: managing genome stability. Int J Biochem Cell Biol 2005; 37(5): 920-926.
  • 16. Rooij DG, Kant HJ, Dol R, Wagemaker G, van Buul PP, van Duijn-Goedhart A, de Jong FH, Broerse JJ. Long term effects of irrradiation before adulthood on reproductive function in the male Rhesus monkey. Biol Reprod 2002; 66(2): 486-494.
  • 17. Johnsen SG. Testicular biopsy score count- a method for registration of spermatogenesis in human testes: normal values and results of 335 hypogonadal males. Hormones 1970; 1(1): 2-25.
  • 18. Akbal C, Tanıdır Y, Turkeri L. Testis kanseri ve infertilite. Üroonkoloji Bülteni 2006; 4: 8-11.
  • 19. Otala M, Suomalainen L, Pentikäinen MO, Kovanen P, Tenhunen M, Erkkila K, Toppari J, Dunkel L. Protection from radiation-induced male germ cell loss by Sphingosine-1-Phosphate. Biol Reprod 2003; 70(3): 759-767.
  • 20. Schally AV, Paz-Bouza JI, Schlosser JV, Karashima T, Debeljuk L, Gandle B, Sampson M. Protective effects of analogs of luteinizing hormone-releasing hormone against x-radiation-induced testicular damage in rats. Proc. Nati. Acad. Sci 1987; 84(3): 851-855.
  • 1. Coşkun Ö. İyonize radyasyonun biyolojik etkileri. SDU Tecnical Sciences Vocational School 2011; 1(2): 13-17.
  • 21. Makker K, Agarwal A, Sharma R. Oxidative stress and male infertility. Indian J Med Res 2009; 129(4): 357-367.
  • 22. Sebastiaa N, Montorob A, Montoroa A, Almonacidb M, Villaescusab JI, C. Jose, Suchc E, Sillac MA, Sorianoa JM, Assessment in vitro of radioprotective efficacy of curcumin and resveratrol. Radiat Meas 2011; 46(9): 962-966.
  • 23. Noorafshan A, Karbalay Doust S, Valizadeh A, Aliabadi E, Mirkhani H. Ameliorative effects of curcumin on the seminiferous epithelium in metronidazole-treated mice: a stereological study. Toxicol Pathol 2010; 38(3): 366-371.
  • 24. Shetty G, Wilson G, Hardy MP, Niu E, Huhtaniemi I, Meistrich ML. Inhibition of recovery of spermatogenesis in irradiated rats by different androgens. Endocrinology 2002; 143(9): 3385-3396.
  • 25. Meistrich ML, Kangasniemi M. Hormone treatment after irradiation stimulates recovery of rat spermatogenesis from surviving spermatogonia. J Androl 1997; 18(1): 80-87.
  • 26. Nalca Andrieu M, Kurtman MC, Hicsonmez A, Özbilgin K, Eser E, Erdemli E. In vivo study to evaluate the protective effects of amifostine on radiation-induced damage of testis tissue. Oncology 2005; 69(1): 44-51.
  • 27. Peltola V, Parvinen M, Huhtaniemi I, Kulmala J, Ahotupa M. Comparison of effects of 0.5 and 3.0 Gy X-irradiation on lipid peroxidation and antioxidant enzyme function in rat testis and liver. J Androl 1993; 14(4): 267-274.
  • 28. Hussein MR, Abu-Dief EE, Abou El-Ghait A, Adly MA, Abdelraheem MH. Morphological evaluation of the radioprotective effects of melatonin against x-ray-induced early and acute testis damage in Albino rats: animal model. Int J Exp Path 2006; 87(3): 237-250.
  • 29. Sharma P, Singh R. Protective role of curcumin on lindane induced reproductive toxicity in male Wistar rats. Bull Environ Contam Toxicol 2010; 84(4): 378–384.
  • 30. Inano H, Onoda M, Inafuku N, Kubota M, Kamada Y, Osawa T, Kobayashi H, Wakabayashi K. Potent preventive action of curcumin on radiation-induced initiation of mammary tumorigenesis in rats. Carcinogenesis 2000; 21(10):1835-1841.
  • 2. Eker L, Acar AO, Demirkan N. Hastaların radyasyon üzerindeki bilgi düzeyleri. MYO-OS 2010- Ulusal Meslek Yüksekokulları Öğrenci Sempozyumu, Düzce. 2010; 21-22.
  • 31. Kanter M, Aktas C, Erboga M. Curcumin attenuates testicular damage, apoptotic germ cell death, and oxidative stress in streptozotocin-induced diabetic rats. Mol. Nutr. Food Res 2012; 56:1-8.
  • 32. Sharaf HA, Morsy FA, Shaffie NM, El-Shennawy ATM. Histological and histochemical study on the protective effect of curcumin on ultraviolet irradiation induced testicular damage in albino rats. J Cytol Histol 2012; 3: 6.
  • 33. Celik-Ozenci C, Tasatargil A, Tekcan M, Sati L, Gungor E, Isbir M, Demir R. Effects of abamectin exposure on male fertility in rats: potential role of oxidative stress-mediated poly(ADP-ribose) polymerase (PARP) activation. Regul Toxicol Pharmacol 2011; 61(3):310-317.
  • 34. Di Meglio S, Denegri M, Vallefuoco S, Tramontano F, Scovassi AI, Quesada P. Poly(ADPR) polymerase-1 and poly(ADPR) glycohydrolase level and distribution in differentiating rat germinal cells. Mol Cell Biochem 2003; 248(1-2):85-91.
  • 35. Tramontano F, Di Meglio S, Quesada P. Co-localization of poly(ADPR)polymerase 1 (PARP-1) poly(ADPR) polymerase 2 (PARP-2) and related proteins in rat testis nuclear matrix defined by chemical cross-linking. J Cell Biochem 2005; 94(1):58-66.
  • 36. Tekcan M, Koksal IT, Tasatargil A, Kutlu O, Gungor E, Celik-Ozenci C. Potential role of poly(ADP-ribose) polymerase activation in the pathogenesis of experimental left varicocele. J Androl 2012; 33(1):122-132.
  • 37. Maymon BB, Cohen-Armon M, Yavetz H, Yogev L, Lifschitz-Mercer B, Kleiman SE, Botchan A, Hauser R, Paz G. Role of poly(ADP ribosyl)ation during human spermatogenesis. Fertil Steril 2006; 86(5):1402-1407.
  • 3. Take G, Erdogan D, Helvacioglu F, Göktas G, Ozbey G, Uluoglu C, Yücel B, Guney Y, Hicsonmez A, Ozkan S. Effect of melatonin and time of administration on irradiation induced damage to rat testes. Braz J Med Biol Res 2009; 42: 621-628.
  • 4. Karakoyun Ö, Aras A, Tuğan D, Hekimgil M, Yalman D, Esassolak M, Haydaroğlu A. The relationship between radiation-induced apoptosis in rat germ cells and amifosine. T Klin J Med Sci 2004;24: 142-146.
  • 5. Zhang Z, Shao S, Meistrich ML. The radiation induced block in spermatogonial differentiation is due to damage to the somatic environment, not the germ cells. J. Cell. Physiol 2007; 211(1): 149-158.
  • 6. Oakberg EF. Sensitivity and time of degeneration of spermatogenic cells irradiated in various stages of maturation in the mouse. Rad Res 1955; 2: 369-391.
  • 7. Aslam I, Fishel S, Moore H, Dowell K, Thornton S. Fertility preservation of boys undergoing anti-cancer therapy: a review of the existing situation and prospects for the future. Hum Reprod 2000; 15(10): 2154-2159.
  • 8. Akpolat M, Topçu Tarladaçalışır Y, Uz YH, Sapmaz Metin M, Kızılay G. Kanser tedavisinde curcuminin yeri. Yeni Tıp Dergisi 2010; 27: 142-147.
  • 9. Kanter M, Topçu Tarladaçalışır Y, Akpolat M, Mercantepe T. Gamma radyasyona bağlı oluşan jejunum mukoza hasarına karşı curcumin ve amifostinin koruyucu etkilerinin incelenmesi. Tıp Araştırmaları Dergisi 2008; 6(3): 128-135.
Year 2018, Volume: 25 Issue: 4, 370 - 384, 01.12.2018
https://doi.org/10.17343/sdutfd.387496

Abstract

References

  • 10. Akpolat M, Kanter M, Uzal MC. Protective effects of curcumin against gamma radiation-induced ileal mucosal damage. Arch Toxicol 2009; 83(6): 609-617.
  • 11. Akpolat M, Topçu-Tarladaçalışır Y, Uz YH, Sapmaz-Metin M, Kızılay G. Kanser Tedavisinde Curcuminin Yeri. Yeni Tıp Dergisi 2010; 27: 142-147.
  • 12. Çimen Özgen S, Dökmeci D, Akpolat M, Karadağ ÇH, Gündüz Ö, Erbaş H, Benian Ö, Uzal C, Turan FN. The Protective Effect of Curcumin on Ionizing Radiation-induced Cataractogenesis in Rats. Balkan Med J 2012; 29: 358-363.
  • 13. Topçu-Tarladaçalışır Y, Akpolat M, Uz YH, Kızılay G, Sapmaz-Metin M, Cerkezkayabekir A, Kurt-Omurlu I. Effects of Curcumin on Apoptosis and Oxidoinflammatory Regulation in Rat Model of Acetic Acid-induced Colitis: Role of JNK and p38 MAPK. Journal of Medicinal Food 2013; 16(4): 296-305. 14. Schreiber V, Ame JC, Dolle P. Poly(ADP-ribose) polymerase-2 (PARP-2) is required for efficient base excision DNA repair in association with 78PARP-1 and XRCC1. J Biol Chem 2002; 277(25): 23028-23036.
  • 15. Meyer-Ficca ML, Meyer RG, Jacobson EL, Jacobson MK. Poly(ADP-ribose) polymerases: managing genome stability. Int J Biochem Cell Biol 2005; 37(5): 920-926.
  • 16. Rooij DG, Kant HJ, Dol R, Wagemaker G, van Buul PP, van Duijn-Goedhart A, de Jong FH, Broerse JJ. Long term effects of irrradiation before adulthood on reproductive function in the male Rhesus monkey. Biol Reprod 2002; 66(2): 486-494.
  • 17. Johnsen SG. Testicular biopsy score count- a method for registration of spermatogenesis in human testes: normal values and results of 335 hypogonadal males. Hormones 1970; 1(1): 2-25.
  • 18. Akbal C, Tanıdır Y, Turkeri L. Testis kanseri ve infertilite. Üroonkoloji Bülteni 2006; 4: 8-11.
  • 19. Otala M, Suomalainen L, Pentikäinen MO, Kovanen P, Tenhunen M, Erkkila K, Toppari J, Dunkel L. Protection from radiation-induced male germ cell loss by Sphingosine-1-Phosphate. Biol Reprod 2003; 70(3): 759-767.
  • 20. Schally AV, Paz-Bouza JI, Schlosser JV, Karashima T, Debeljuk L, Gandle B, Sampson M. Protective effects of analogs of luteinizing hormone-releasing hormone against x-radiation-induced testicular damage in rats. Proc. Nati. Acad. Sci 1987; 84(3): 851-855.
  • 1. Coşkun Ö. İyonize radyasyonun biyolojik etkileri. SDU Tecnical Sciences Vocational School 2011; 1(2): 13-17.
  • 21. Makker K, Agarwal A, Sharma R. Oxidative stress and male infertility. Indian J Med Res 2009; 129(4): 357-367.
  • 22. Sebastiaa N, Montorob A, Montoroa A, Almonacidb M, Villaescusab JI, C. Jose, Suchc E, Sillac MA, Sorianoa JM, Assessment in vitro of radioprotective efficacy of curcumin and resveratrol. Radiat Meas 2011; 46(9): 962-966.
  • 23. Noorafshan A, Karbalay Doust S, Valizadeh A, Aliabadi E, Mirkhani H. Ameliorative effects of curcumin on the seminiferous epithelium in metronidazole-treated mice: a stereological study. Toxicol Pathol 2010; 38(3): 366-371.
  • 24. Shetty G, Wilson G, Hardy MP, Niu E, Huhtaniemi I, Meistrich ML. Inhibition of recovery of spermatogenesis in irradiated rats by different androgens. Endocrinology 2002; 143(9): 3385-3396.
  • 25. Meistrich ML, Kangasniemi M. Hormone treatment after irradiation stimulates recovery of rat spermatogenesis from surviving spermatogonia. J Androl 1997; 18(1): 80-87.
  • 26. Nalca Andrieu M, Kurtman MC, Hicsonmez A, Özbilgin K, Eser E, Erdemli E. In vivo study to evaluate the protective effects of amifostine on radiation-induced damage of testis tissue. Oncology 2005; 69(1): 44-51.
  • 27. Peltola V, Parvinen M, Huhtaniemi I, Kulmala J, Ahotupa M. Comparison of effects of 0.5 and 3.0 Gy X-irradiation on lipid peroxidation and antioxidant enzyme function in rat testis and liver. J Androl 1993; 14(4): 267-274.
  • 28. Hussein MR, Abu-Dief EE, Abou El-Ghait A, Adly MA, Abdelraheem MH. Morphological evaluation of the radioprotective effects of melatonin against x-ray-induced early and acute testis damage in Albino rats: animal model. Int J Exp Path 2006; 87(3): 237-250.
  • 29. Sharma P, Singh R. Protective role of curcumin on lindane induced reproductive toxicity in male Wistar rats. Bull Environ Contam Toxicol 2010; 84(4): 378–384.
  • 30. Inano H, Onoda M, Inafuku N, Kubota M, Kamada Y, Osawa T, Kobayashi H, Wakabayashi K. Potent preventive action of curcumin on radiation-induced initiation of mammary tumorigenesis in rats. Carcinogenesis 2000; 21(10):1835-1841.
  • 2. Eker L, Acar AO, Demirkan N. Hastaların radyasyon üzerindeki bilgi düzeyleri. MYO-OS 2010- Ulusal Meslek Yüksekokulları Öğrenci Sempozyumu, Düzce. 2010; 21-22.
  • 31. Kanter M, Aktas C, Erboga M. Curcumin attenuates testicular damage, apoptotic germ cell death, and oxidative stress in streptozotocin-induced diabetic rats. Mol. Nutr. Food Res 2012; 56:1-8.
  • 32. Sharaf HA, Morsy FA, Shaffie NM, El-Shennawy ATM. Histological and histochemical study on the protective effect of curcumin on ultraviolet irradiation induced testicular damage in albino rats. J Cytol Histol 2012; 3: 6.
  • 33. Celik-Ozenci C, Tasatargil A, Tekcan M, Sati L, Gungor E, Isbir M, Demir R. Effects of abamectin exposure on male fertility in rats: potential role of oxidative stress-mediated poly(ADP-ribose) polymerase (PARP) activation. Regul Toxicol Pharmacol 2011; 61(3):310-317.
  • 34. Di Meglio S, Denegri M, Vallefuoco S, Tramontano F, Scovassi AI, Quesada P. Poly(ADPR) polymerase-1 and poly(ADPR) glycohydrolase level and distribution in differentiating rat germinal cells. Mol Cell Biochem 2003; 248(1-2):85-91.
  • 35. Tramontano F, Di Meglio S, Quesada P. Co-localization of poly(ADPR)polymerase 1 (PARP-1) poly(ADPR) polymerase 2 (PARP-2) and related proteins in rat testis nuclear matrix defined by chemical cross-linking. J Cell Biochem 2005; 94(1):58-66.
  • 36. Tekcan M, Koksal IT, Tasatargil A, Kutlu O, Gungor E, Celik-Ozenci C. Potential role of poly(ADP-ribose) polymerase activation in the pathogenesis of experimental left varicocele. J Androl 2012; 33(1):122-132.
  • 37. Maymon BB, Cohen-Armon M, Yavetz H, Yogev L, Lifschitz-Mercer B, Kleiman SE, Botchan A, Hauser R, Paz G. Role of poly(ADP ribosyl)ation during human spermatogenesis. Fertil Steril 2006; 86(5):1402-1407.
  • 3. Take G, Erdogan D, Helvacioglu F, Göktas G, Ozbey G, Uluoglu C, Yücel B, Guney Y, Hicsonmez A, Ozkan S. Effect of melatonin and time of administration on irradiation induced damage to rat testes. Braz J Med Biol Res 2009; 42: 621-628.
  • 4. Karakoyun Ö, Aras A, Tuğan D, Hekimgil M, Yalman D, Esassolak M, Haydaroğlu A. The relationship between radiation-induced apoptosis in rat germ cells and amifosine. T Klin J Med Sci 2004;24: 142-146.
  • 5. Zhang Z, Shao S, Meistrich ML. The radiation induced block in spermatogonial differentiation is due to damage to the somatic environment, not the germ cells. J. Cell. Physiol 2007; 211(1): 149-158.
  • 6. Oakberg EF. Sensitivity and time of degeneration of spermatogenic cells irradiated in various stages of maturation in the mouse. Rad Res 1955; 2: 369-391.
  • 7. Aslam I, Fishel S, Moore H, Dowell K, Thornton S. Fertility preservation of boys undergoing anti-cancer therapy: a review of the existing situation and prospects for the future. Hum Reprod 2000; 15(10): 2154-2159.
  • 8. Akpolat M, Topçu Tarladaçalışır Y, Uz YH, Sapmaz Metin M, Kızılay G. Kanser tedavisinde curcuminin yeri. Yeni Tıp Dergisi 2010; 27: 142-147.
  • 9. Kanter M, Topçu Tarladaçalışır Y, Akpolat M, Mercantepe T. Gamma radyasyona bağlı oluşan jejunum mukoza hasarına karşı curcumin ve amifostinin koruyucu etkilerinin incelenmesi. Tıp Araştırmaları Dergisi 2008; 6(3): 128-135.
There are 36 citations in total.

Details

Primary Language Turkish
Subjects Clinical Sciences
Journal Section Research Articles
Authors

Meryem Akpolat

Bayram Kamat This is me

Kanat Gülle

Bekir Hakan Bakkal This is me

Publication Date December 1, 2018
Submission Date January 31, 2018
Acceptance Date February 21, 2018
Published in Issue Year 2018 Volume: 25 Issue: 4

Cite

Vancouver Akpolat M, Kamat B, Gülle K, Bakkal BH. Skrotal Radyoterapide Curcuminin Profilaktik Kullanımı Testis Dokusunda PARP-1 İmmünreaktivitesini ve Spermatogenezi Nasıl Etkiler?. Med J SDU. 2018;25(4):370-84.

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