The study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant protein production. Continuous culture is the most common method used to grow cells for recombinant protein production. In the study, the K12 strain of E. coli was used as a culture for the production of Expansin protein. The used LeExp1 gene was isolated from a young tomato plant. Since the related gene is found in very small amounts in plants, it has been reproduced using the PCR method and has been made workable with this method. T17 vector (T7 RNA polymerase system), which is frequently used in the production of recombinant protein, was used as the bacterial expression vector. The T7 RNA polymerase system is a commonly used vector in E. coli. With the transfer, the E. coli bacterium was given the ability to produce recombinant protein. Whether the obtained recombinant protein expressed the appropriate protein was determined by SDS Page analysis.
Recombinant protein Expansin protein LeExp1 gene Escherichia coli PCR
TÜBİTAK
113O392
Researchers would like to thank TÜBİTAK for its support.
113O392
Birincil Dil | İngilizce |
---|---|
Konular | Gıda Mühendisliği |
Bölüm | Makaleler |
Yazarlar | |
Proje Numarası | 113O392 |
Yayımlanma Tarihi | 27 Mart 2023 |
Gönderilme Tarihi | 17 Ekim 2022 |
Kabul Tarihi | 14 Aralık 2022 |
Yayımlandığı Sayı | Yıl 2023 Cilt: 7 Sayı: 1 |
International Journal of Agriculture, Environment and Food Sciences dergisinin içeriği, Creative Commons Alıntı-GayriTicari (CC BY-NC) 4.0 Uluslararası Lisansı ile yayınlanmaktadır. Söz konusu telif, üçüncü tarafların içeriği uygun şekilde atıf vermek koşuluyla, ticari olmayan amaçlarla paylaşımına ve uyarlamasına izin vermektedir. Yazarlar, International Journal of Agriculture, Environment and Food Sciences dergisinde yayınlanmış çalışmalarının telif hakkını elinde tutar.
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